Journal: bioRxiv

Article Title: Acquired resistance to the PRMT5 inhibitor confers collateral sensitivity to MEK inhibition in MTAP-null non-small cell lung cancer

doi: 10.64898/2026.04.16.719008

Figure Lengend Snippet: High-throughput drug screen and MEK inhibitor sensitivity in MRTX1719-resistant NSCLC cells. (A) Composition of the compound library used for drug screen, including SGC epigenetic compounds, FDA-approved oncology drugs, and TargetMol epigenetic inhibitors. (B) IC50 values of MRTX1719 and anisomycin in DMSO and MRTXR cells. Anisomycin was included as a nonselective control in the drug screen. (C) Dose-response curves of DMSO and MRTXR cells treated with the MEK inhibitor selumetinib. Data are presented as mean ± SD. (D) Synergy heatmaps of MRTX1719 and selumetinib in DMSO and MRTXR cells. Synergy mean scores were calculated using the Bliss model with the SynergyFinder+ tool.

Article Snippet: The screening library comprised 619 compounds, including SGC epigenetic compounds, TargetMol epigenetic inhibitors, and FDA-approved oncology drugs ( ).

Techniques: High Throughput Screening Assay, Drug discovery, Control

Journal: Nutrients

Article Title: Microbiota Transplantation in Individuals with Type 2 Diabetes and a High Degree of Insulin Resistance

doi: 10.3390/nu16203491

Figure Lengend Snippet: ( 1a ) Results of the 75 g oral glucose tolerance test (OTTG) regarding glucose in the placebo group. ( 1b ) Results of the 75 g oral glucose tolerance test (OTTG) regarding glucose in the probiotic treatment group. ( 1c ) Results of the 75 g oral glucose tolerance test (OTTG) regarding glucose in the fecal microbiota transplantation (FMT) group. ( 2a ) Results of the 75 g oral glucose tolerance test (OTTG) regarding insulin in the placebo group. ( 2b ) Results of the 75 g oral glucose tolerance test (OTTG) regarding insulin in the probiotic treatment group. ( 2c ) Results of the 75 g oral glucose tolerance test (OTTG) regarding insulin in the fecal microbiota transplantation (FMT) group.

Article Snippet: This Phase II, randomized, single-blind, parallel-arm clinical trial was performed at Virgen de la Victoria University Hospital in Málaga (Spain), as a sub-study of the project “ Epigenetic modifications and microbiota in the genesis of adipose tissue dysfunction and insulin resistance ” (EPIGEN-MICROBIOTA), and was approved by our local Ethics Committee.

Techniques: Transplantation Assay

Journal: Nutrients

Article Title: Microbiota Transplantation in Individuals with Type 2 Diabetes and a High Degree of Insulin Resistance

doi: 10.3390/nu16203491

Figure Lengend Snippet: ( a ) Number of bacteria species that were lost, maintained, or gained with respect to the baseline profile with each of the interventions: fecal microbiota transplant (FMT), probiotic intervention (PB), or placebo groups at 4 weeks or 12 weeks. ( b ) Within the FMT intervention, the number of bacteria species shared between the donor and the receptor, the number of species only found in the donor, and the number of species only found in the receptor of the intervention in relation to their respective donors at different time points.

Article Snippet: This Phase II, randomized, single-blind, parallel-arm clinical trial was performed at Virgen de la Victoria University Hospital in Málaga (Spain), as a sub-study of the project “ Epigenetic modifications and microbiota in the genesis of adipose tissue dysfunction and insulin resistance ” (EPIGEN-MICROBIOTA), and was approved by our local Ethics Committee.

Techniques: Bacteria

Journal: Current opinion in insect science

Article Title: Recent Advances and Future Perspectives in Vector-omics

doi: 10.1016/j.cois.2020.05.006

Figure Lengend Snippet: Evaluating the pros and cons of selected sequencing and mapping technologies.

Article Snippet: Oxford Nanopore Technologies , Long read sequencing technology used for de novo genome assembly, isoform identification, and detecting epigenetic modifications. , Sequencing of long DNA molecules directly enable detection of epigenetic modifications. Can sequence transcripts for full cDNA and direct RNA sequencing for nucleotide modification detection. Length of reads are only limited by the DNA isolation and sequencing library. Sequencing devices are portable and scalable. Real time analysis is possible for rapid workflows. Several insect tissues have been sequenced showing promise for the vector field. Continuing community-guided improvements to chemistry , High error rate but can be corrected by overlap consensus. Sensitive to long homopolymers. Requires hands-on training to operate. Limited protocols for vectors. Constant updates to kits and user interface which make it challenging for comparison and to keep pace. , ~15 kb/2Mb* (in theory, read length is only limited by the DNA input).

Techniques: Sequencing, Plasmid Preparation, RNA Sequencing Assay, Modification, DNA Extraction, Comparison, Scaffolding, Variant Assay, Produced, Immunoprecipitation, Genome Wide, Expressing, Binding Assay, Activity Assay, Amplification